The fabrication of amperometric biosensors based on whole cell Gluconobacter oxydans DSMZ 2343 (G. oxydans) and glucose oxidase (GOx) was performed for the detection of glucose. Glassy carbon electrodes (GCE) were coated with a 10-(4H-dithiyeno [3,2-b:2’,3’-d]pyroll-4-il)decan-1-amine (DTP-alkyl-NH2) polymer using an electropolymerization method and the formed interface was used to connect the bacteria and the enzyme to the electrode. The transfer of electrons from enzyme to electrode was successfully demonstrated by the biocatalytic activity and unique morphology of the conducting polymer. Characterization of the biosensors was assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM) analyses. The detection limits of the enzyme and microbial based biosensors for glucose were 0.022 and 0.081 mM, respectively. The broad linear dynamic ranges of the GOx and G. oxydans biosensors were observed to be 0.045–50.0 and 0.19–50.0 mM, respectively. The analytical performances of biosensors were compared according to the following figures of merit: detection limits, limits of quantification, pH and current response time. In addition, to demonstrate the applicability of the biosensors, real-time measurements and recovery studies were evaluated.
Eser Adı (dc.title) | Electrochemical Glucose Biosensors: Whole Cell Microbial and Enzymatic Determination Based on 10-(4H-Dithieno[3,2-b:2′,3′-d]Pyrrol-4-yl)Decan-1-Amine Interfaced Glassy Carbon Electrodes |
Yayın Türü (dc.type) | Makale |
Yazar/lar (dc.contributor.author) | ÇEVİK, Emre |
Yazar/lar (dc.contributor.author) | CERİT, Alaaddin |
Yazar/lar (dc.contributor.author) | TOMBULOĞLU, Hüseyin |
Yazar/lar (dc.contributor.author) | SABİT, Hussein |
Yazar/lar (dc.contributor.author) | YILDIZ, Hüseyin Bekir |
Atıf Dizini (dc.source.database) | Wos |
Atıf Dizini (dc.source.database) | Scopus |
Konu Başlıkları (dc.subject) | Glucose Biosensor |
Konu Başlıkları (dc.subject) | Glucose Oxidase |
Konu Başlıkları (dc.subject) | G. Oxydans |
Konu Başlıkları (dc.subject) | 10-(4H-dithiyeno[3,2-b:2′, 3′-d]pyroll-4-il)decan-1-amine (DTP) Conducting Polymer |
Konu Başlıkları (dc.subject) | Whole Cell Microbial Biosensor |
Yayıncı (dc.publisher) | Taylor and Francis |
Yayın Tarihi (dc.date.issued) | 2019 |
Kayıt Giriş Tarihi (dc.date.accessioned) | 2019-07-09T06:03:56Z |
Açık Erişim tarihi (dc.date.available) | 2019-07-09T06:03:56Z |
Özet (dc.description.abstract) | The fabrication of amperometric biosensors based on whole cell Gluconobacter oxydans DSMZ 2343 (G. oxydans) and glucose oxidase (GOx) was performed for the detection of glucose. Glassy carbon electrodes (GCE) were coated with a 10-(4H-dithiyeno [3,2-b:2’,3’-d]pyroll-4-il)decan-1-amine (DTP-alkyl-NH2) polymer using an electropolymerization method and the formed interface was used to connect the bacteria and the enzyme to the electrode. The transfer of electrons from enzyme to electrode was successfully demonstrated by the biocatalytic activity and unique morphology of the conducting polymer. Characterization of the biosensors was assessed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM) analyses. The detection limits of the enzyme and microbial based biosensors for glucose were 0.022 and 0.081 mM, respectively. The broad linear dynamic ranges of the GOx and G. oxydans biosensors were observed to be 0.045–50.0 and 0.19–50.0 mM, respectively. The analytical performances of biosensors were compared according to the following figures of merit: detection limits, limits of quantification, pH and current response time. In addition, to demonstrate the applicability of the biosensors, real-time measurements and recovery studies were evaluated. |
Yayın Dili (dc.language.iso) | en |
Sponsor-Yayıncı (dc.description.sponsorship) | The authors would like to thank the European Union through the COST Action CM1104 “Reducible Oxide Chemistry, Structure and Functions” and the Scientific and Technological Research Council of Turkey (TUBITAK Grant Number 112T622) for the financial support of this work. |
Tek Biçim Adres (dc.identifier.uri) | https://hdl.handle.net/20.500.12498/721 |