Aim: The development of calixarene based phenol biosensor. Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy inter-action with Au surface and one-step biomolecule immo-bilization. Self-assembled monolayers (SAMs) of p-amino-functionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) nzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2/Lac) was used for the electrochemical anal-ysis of phenolic compounds at − 50 mV. Results: The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was inves-tigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step. Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic con-taminations in samples.
Eser Adı (dc.title) | A Novel Immobilization Matrix For The Biosensing Of Phenol: Self Assembled Monolayers Of Calixarenes |
Yayın Türü (dc.type) | Makale |
Yazar/lar (dc.contributor.author) | TAŞÇI, Filiz |
Yazar/lar (dc.contributor.author) | SAYIN, Serkan |
Yazar/lar (dc.contributor.author) | AĞ SELECİ, Didem |
Yazar/lar (dc.contributor.author) | DEMİR, Bilal |
Yazar/lar (dc.contributor.author) | AZAK, Hacer |
Yazar/lar (dc.contributor.author) | YILDIZ, Hüseyin Bekir |
Yazar/lar (dc.contributor.author) | ODACI DEMİRKOL, Dilek |
Yazar/lar (dc.contributor.author) | TİMUR, Suna |
Atıf Dizini (dc.source.database) | Wos |
Atıf Dizini (dc.source.database) | Scopus |
Konu Başlıkları (dc.subject) | Functionalized Calixarene |
Konu Başlıkları (dc.subject) | Laccase |
Konu Başlıkları (dc.subject) | Phenol Detection |
Konu Başlıkları (dc.subject) | Amperometric Biosensor |
Konu Başlıkları (dc.subject) | Immobilization of Enzymes |
Yayıncı (dc.publisher) | Turkish Journal of Biochemistry (Turkish Biochemical Society) |
Yayın Tarihi (dc.date.issued) | 2017 |
Kayıt Giriş Tarihi (dc.date.accessioned) | 2019-07-09T06:25:17Z |
Açık Erişim tarihi (dc.date.available) | 2019-07-09T06:25:17Z |
Özet (dc.description.abstract) | Aim: The development of calixarene based phenol biosensor. Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy inter-action with Au surface and one-step biomolecule immo-bilization. Self-assembled monolayers (SAMs) of p-amino-functionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) nzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2/Lac) was used for the electrochemical anal-ysis of phenolic compounds at − 50 mV. Results: The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was inves-tigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step. Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic con-taminations in samples. |
Yayın Dili (dc.language.iso) | en |
Tek Biçim Adres (dc.identifier.uri) | https://hdl.handle.net/20.500.12498/731 |