Comparison of real-time PCR, indirect immunofluorescence antibody assay, and different staining techniques for the diagnosis of Pneumocystis jirovecii

DURGUT, Elif Gözde | KUŞTİMUR, Semra | AYDIN TERZİOĞLU, Merve | KALKANCI, Ayşe | DOĞRUMAN AL, Funda

Aim: Pneumocystis pneumonia (PCP), caused by Pneumocystis jirovecii (P. jirovecii), is an opportunistic infection with a severe progression, often observed in immunocompromised patients. The diagnosis is difficult due to the non-specific clinical and radiological findings. Therefore, rapid and accurate diagnosis of the agent is important in terms of timely implementation of the treatment. In this study, it was aimed to detect P. jirovecii by Giemsa staining, Modified Toluidine Blue O staining (MTolB), indirect immunofluorescent antibody (IIFA) assay, and real-time polymerase chain reaction (PC ...Daha fazlası