Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase

ÇEVİK, Emre | CERİT, Alaaddin | GAZEL, Nilay | YILDIZ, Hüseyin Bekir

In this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2’,3’d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances, linear range, de ...Daha fazlası