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TAŞÇI, Filiz | SAYIN, Serkan | AĞ SELECİ, Didem | DEMİR, Bilal | AZAK, Hacer | YILDIZ, Hüseyin Bekir | ODACI DEMİRKOL, Dilek | TİMUR, Suna
Aim: The development of calixarene based phenol biosensor. Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy inter-action with Au surface and one-step biomolecule immo-bilization. Self-assembled monolayers (SAMs) of p-amino-functionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) ...Daha fazlası
ÇEVİK, Emre | CERİT, Alaaddin | TOMBULOĞLU, Hüseyin | SABİT, Hussein | YILDIZ, Hüseyin Bekir
The fabrication of amperometric biosensors based on whole cell Gluconobacter oxydans DSMZ 2343 (G. oxydans) and glucose oxidase (GOx) was performed for the detection of glucose. Glassy carbon electrodes (GCE) were coated with a 10-(4H-dithiyeno [3,2-b:2’,3’-d]pyroll-4-il)decan-1-amine (DTP-alkyl-NH2) polymer using an electropolymerization method and the formed interface was used to connect the bacteria and the enzyme to the electrode. The transfer of electrons from enzyme to electrode was successfully demonstrated by the biocatalytic activity and unique morphology of the conducting polymer. Ch ...Daha fazlası
AZAK, Hacer | KURBANOĞLU, Sevinç | YILDIZ, Hüseyin Bekir | ÖZKAN, Sibel Ayşıl
The synthesis of derivatives of dithionepyrrole is a hot subject that has been studied extensively. In this study, a novel approach for constructing different glucose biosensors using conducting polymers of 4-(4H-dithyinol[3,2-b:2′,3′-d]pyroll-4-yl)aniline and 4-(4H-dithyinol[3,2-b:2′,3′-d]pyroll-4-yl)-[1,1′-biphenyl]-4-amine is proposed. After the synthesis and characterizations of the dithionepyrrole type monomers, they were used in glucose biosensing applications. The surface of gold electrode was modified with mercaptoethane sulfonic acid and p-aminothiophenol functionalized gold nanoparti ...Daha fazlası
AZAK, Hacer | YILDIZ, Hüseyin Bekir | ÇARBAŞ, Buket
Synthesis and electropolymerization of a new conjugated dithieno(3,2-b:20,30-d) pyrole (DTP) derivative, namely, 2-(2-(2-(4H-dithieno[3,2-b:20,30-d]pyrrol-4-yl)ethoxy)ethoxy)ethanamine, (DTP-alkoxy-NH2) monomer are presented. The electrochemical, spectroelectrochemical effects and biosensor capability towards to glucose oxidase enzyme of P(DTP-alkoxy-NH2) polymer fi lm are investigated in detail during the placement of alkoxy-NH2 functional unit in the polymer. Electrochemical and optical results between neutral and oxidized states of the polymer fi lm show that (P(DTP-alkoxy-NH2)) has reversibl ...Daha fazlası
DERVISEVIC, Muamer | DERVISEVIC, Esma | AZAK, Hacer | ÇEVİK, Emre | ŞENEL, Mehmet | YILDIZ, Hüseyin Bekir
In this paper, a novel amperometric xanthine (X) biosensor is constructed by xanthine oxidase (XOx) immobilization on the pencil graphite electrode (PGE). Xanthine oxidase is immobilized using glutaraldehyde (GA) on the electrochemically polymerized conducting polymer film. The detection of xanthine is based on its consumed amount due to the enzymatic reaction of xanthine oxidase. The effects of polymer thickness, applied potential, pH, and temperature were investigated and optimum parameters were found to be five cycles, +5 V, +0.5 V and 30 °C, respectively. Storage stability, operation stabi ...Daha fazlası
Enzyme technology has received great attention with improvements in biological remediation techniques. Enzyme-based biosensors can qualify as a measuring system as they permit the detection of biological events by detecting biochemical changes in biological processes. Advantages of biosensors are identification of even small quantities, cost effectiveness, and requirement of less power and less volume for identifying a wide linear range for usage [1]. Moreover, biosensors have high sensitivity, selectivity, and good response time for different kinds of applications.
ERYÜRÜK, Şule | NESİMİOĞLU, Barış Samim | YILDIZ, Hüseyin Bekir | ALTUN, Hüseyin Oktay | YUMUŞAK, Semih | AÇIKGÖZ, Hulusi | BAĞRIYANIK, Ozan İbrahim Ethem | ÇALIK, Ahmet
In Turkey, agricultural manufacturing plays a big role in industry as a sector and can produce almost all categories of agricultural equipment. When considered agricultural machinery sector, it is ranked as the 5th biggest machinery sector in Turkey, covering 7% of the total exports and in domestic market, agricultural machinery sector is ranked as 4th sector. From a regional point of view, it can be stated that İstanbul, Ankara, İzmir, Konya, AydIn, Manisa and BalIkesir regions are prominent in the sector in terms of both manufacturing volume and number of employments. However, when it comes ...Daha fazlası
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve cerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.