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Bulunan: 6 Adet 0.001 sn
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Süresiz Ambargo

Identification of medically important yeasts by sequence analysis of the internal transcribed spacer and D1/D2 region of the large ribosomal subunit

AYDIN, Merve | KUŞTİMUR, Semra | KALKANCI, Ayşe | DURAN, Tuğçe

Background: The prevalence of opportunistic yeast infections has increased in recent decades as the resultof an increasing immunocompromised patient population.Aims: To evaluate ribosomal RNA (rRNA) gene sequence to identify medically important yeast species, toinvestigate the performance of both the rRNA gene internal transcribed spacer (ITS) and D1/D2 region in identifying clinically relevant yeasts, and to compare these results with those of a standard phenotypicmethod.Methods: Both regions from 50 yeast strains, comprising 45 clinical isolates and 5 reference strains, wereamplified using P ...Daha fazlası

Erişime Açık

Molecular characterization and subtyping of Blastocystis in urticarial patients in Turkey

AYDIN, Merve | YAZICI, Mustafa | DEMİRKAZIK, Mehtap | KOLTAŞ, İsmail Soner | ÇIKMAN, Aytekin | GÜLHAN, Barış | DURAN, Tuğçe | YILMAZ, Aysun | KARA, Murat

Objective: To investigate Blastocystis’ etiologic role and association with gastrointestinal symptomatology in acute and chronic urticaria patients and to identify Blastocystis subtypes responsible for urticaria. Methods: The study included urticaria patients and healthy individuals that presented to our polyclinic between June 2015 and May 2017. The participants were assigned into Group I (137 patients), subdivided into acute (72) and chronic urticaria patients (65), and Group II (129 control individuals). Blastocystis presence was investigated by native-Lugol examination, trichrome staining, ...Daha fazlası

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Nadir Görülen Bir Vaka: Fanconi Aplastik Anemisi Gelişen Bir Klinefelter Sendromu Olgusu

TOKGÖZ, Hüseyin | ÇALIŞKAN, Ümran | DURAN, Tuğçe | ÇALIŞKAN SAK, Kaniye Zeynep

Giriş: Fankoni Aplastik Anemisi, çeşitli konjenital malformasyonlar, progresif pansitopeni ve genellikle hematolojik malignitelere yatkınlık ile karakterize genetik olarak çoğunlukla otozomal resesif olarak geçen bir hastalıktır. Konjenital anomaliler hastadan hastaya değişir ve başta iskelet malformasyonlarının yanı sıra ana organ sistemlerinden herhangi birini etkileyebilir. Klinefelter sendromu erkek hipogonadizminin en yaygın genetik formudur ve en sık görülen karyotip 47, XXY'dir. Vaka Sunumu: Halsizlik ve solukluk şikayetleri ile başvuran 9 yaşında erkek hastanın fizik muayenesinde kilo, ...Daha fazlası

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Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey.

GÜLHAN, Barış | AYDIN TERZİOĞLU, Merve | DEMİRKAZIK, Mehtap | KOLTAŞ, İsmail Soner | ÇIKMAN, Aytekin | TÜRKMEN, Kültigin | DURAN, Tuğçe

Introduction: The aim of this study was to determine the Blastocystis prevalence and subtypes in hemodialysis patients in Turkey. Methodology: Eighty-four patients diagnosed with end-stage renal failure who were undergoing hemodialysis and 20 healthy volunteers were enrolled. Blastocystis presence was investigated by native-Lugol, trichrome staining, PCR using STS primers, and DNA sequencing analysis. Results: Among the stool samples from the hemodialysis patients, 9.52% (8/84) were found to be Blastocystis-positive with the native-Lugol and trichrome staining. Seven of the eight Blastocystis ...Daha fazlası

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In vitro antifungal and antibiofilm activities of novel sulfonyl hydrazone derivatives against Candida spp.

AYDIN TERZİOĞLU, Merve | ÖZTÜRK, Ali | DURAN, Tuğçe | ÖZMEN, Ümmühan Özdemir | ŞUMLU, Esra | AYAN, Esra Bilen | KORUCU, Emine Nedime

Background: The aim of this study was to investigate the antifungal and antibiofilm activity of the new sulfonyl hydrazones compound derived from sulphonamides. Methods: In this study, new sulfonyl hydrazone series were synthesized via a green chemistry method. The structures of the synthesized compounds were characterized by elemental analyses and spectroscopic methods. The antifungal activities of the Anaf compounds against Candida strains under planktonic conditions were tested. The biofilm-forming ability of Candida strains was determined and the inhibitory effects of Anaf compounds on Can ...Daha fazlası

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Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

TOKAK, Semih | KILIÇ, İbrahim Halil | YALÇIN, Hüsniye Tansel | DURAN, Tuğçe

Yeasts are usually found in high amounts in dairy products, which show their ability to adapt to substrates rich in protein, lipid. sugar and organic acids. A wide distribution of yeast in dairy products is a result of proteolytic and lipolytic is activities. Spoilage yeasts and molds can grow in most. processed and raw limits. where environmental conditions are not suitable for most bacteria (low pH low water activity, aw). Nutrients and oxygen in food are the main factors that determine the type of fungal spoilage. In this study, dairy products samples (yoghurt, cream. butter, curd cheese. A ...Daha fazlası

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