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Calixarene modified montmorillonite: A novel design for biosensing applications

Sonmez, B.; Sayin, S.; Yalcinkaya, E.E.; Seleci, D.A.; Yildiz, H.B.; Demirkol, D.O.; Timur, S.
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Date
2014
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http://hdl.handle.net/20.500.12498/3139
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Abstract
Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH<inf>2</inf>) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric anaalysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH<inf>2</inf> modified Mt (Calix-NH<inf>2</inf>/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a Calix-NH<inf>2</inf>/Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail. © 2014 The Royal Society of Chemistry....  Show more  Show less
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