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Folic-Acid-Modified Conducting Polymer: Electrochemical Detection of the Cell Attachment.

Azak, Hacer; Barlas, Firat Baris; Yildiz, Huseyin Bekir; Gulec, Kadri; Demir, Bilal; Demirkol, Dilek Odaci; Timur, Suna
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Date
2016-04
URI
http://hdl.handle.net/20.500.12498/3343
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Abstract
Here, postfunctionalization and bioapplication of a π-conjugated polymer named 4-[4H-dithieno(3,2-b:2',3'-d)pyrrol-4-yl]aniline (DTP-aryl-NH2 ) are reported, which is successfully synthesized via electropolymerization onto the glassy carbon electrode. Folic acid (FA) is used to modify the amino functional polymer via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide chemistry for the further steps. The selective adhesion of folate receptor positive cells on the suurface is followed by the electrochemical methods. Cyclic voltammetry and electrochemical impedance spectroscopy have been used to characterize stepwise modification of the electroactive surface. After optimization studies such as scan rate during the polymer deposition, FA amount for the efficient surface targeting, incubation time with the cells etc., analytical characterization is carried out. The surface morphologies at each step are imaged by using fluorescence microscopy....  Show more  Show less
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