Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase
Cevik, Emre and Cerit, Alaaddin and Gazel, Nilay and Yildiz, Huseyin
Bekir
Loading
Abstract
In this study, an amperometric cholesterol biosensor was constructed
based on cholesterol oxidase immobilized on a conducting
4-(4H-dithienol{[}3,2-b:2,3-d]pyrrole-4)aniline polymer,
(DTP(aryl)aniline). Glassy carbon electrodes were covered with
P(DTP(aryl)aniline) which is used for the wiring of enzyme to the
electrode surface by using electro-polymerization. The electron transfer
was successfully made by the bio-catalytic activity and possession of
the unique morphology of the polymer allowed efficient immobilization of
the cholesterol oxidase enzyme. Analytical performances; linear range,
detection limit, limit of quantification and the Michaelis-Menten
constant (K-m) of biosensor electrodes were obtained 2.0M-23.7M, 0.27M,
0.82M, 17,81M respectively. Biosensor optimization parameters: optimum
pH, optimum temperature, stability test and response time were
evaluated. The real sample and recovery studies were also performed in
order to show applicability of the biosensing electrodes.... Show more Show less