The effects of aflatoxin B-1 on growth hormone regulated gene-1 and interaction between DNA and aflatoxin B-1 in broiler chickens during hatching
Mutlu, E. Gulbahce and Arslan, E. and Oznurlu, Y. and Ozparlak, H.
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Abstract
Many types of aflatoxin cause problems for both public and animal
health. Aflatoxin B-1 (AFB(1)) is the most toxic and commonly
encountered fungal toxin that appears in poultry feed and in feeds
stored under unsuitable conditions. AFB(1) decreases feed quality, egg
production and fertility of hatching eggs. Also, AFB(1) alters the
development of embryos by infecting eggs. We investigated using sequence
analysis the changes caused by different concentrations of AFB(1) on the
promoter sequences off the growth hormone regulated gene-1 (GHRG-1) in
chick embryo at 13, 17, 19 and 21days incubation. DNA isolated from the
liver of chick embryos treated with different concentrations of AFB(1)
was separated using agarose gel electrophoresis to detect apoptosis, and
DNA interaction with AFB(1) was investigated using plasmids to detect
changes in electrophoretic mobility and their effects on DNA. Base
changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and
40 ng/egg doses of AFB(1) were increased on day 19 compared to base
changes of the same AFB(1) doses on day 13. We also found that AFB at
different concentrations changed the mobility of DNA by binding to it,
and that high doses of AFB1 destroyed DNA. The DNA interaction study
using plasmid demonstrated that AFB(1) at high doses was bound to
plasmid DNA, slowed its mobility and inhibited restriction cuts.... Show more Show less