MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing
Menger, Marcus and Yarman, Aysu and Erdossy, Julia and Yildiz, Huseyin
Bekir and Gyurcsanyi, Robert E. and Scheller, Frieder W.
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Abstract
Biomimetic binders and catalysts have been generated in order to
substitute the biological pendants in separation techniques and
bioanalysis. The two major approaches use either ``evolution in the test
tube{''} of nucleotides for the preparation of aptamers or total
chemical synthesis for molecularly imprinted polymers (MIPs). The
reproducible production of aptamers is a clear advantage, whilst the
preparation of MIPs typically leads to a population of polymers with
different binding sites. The realization of binding sites in the total
bulk of the MIPs results in a higher binding capacity, however, on the
expense of the accessibility and exchange rate. Furthermore, the readout
of the bound analyte is easier for aptamers since the integration of
signal generating labels is well established. On the other hand, the
overall negative charge of the nucleotides makes aptamers prone to
non-specific adsorption of positively charged constituents of the sample
and the ``biological{''} degradation of non-modified aptamers and ionic
strength-dependent changes of conformation may be challenging in some
application.... Show more Show less