Electrochemical glucose biosensing via new generation DTP type conducting polymers/gold nanoparticles/glucose oxidase modified electrodes
Azak, Hacer and Kurbanoglu, Sevinc and Yildiz, Huseyin Bekir and Ozkan,
Sibel A.
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Abstract
The synthesis of derivatives of dithionepyrrole is a hot subject that
has been studied extensively. In this study, a novel approach for
constructing different glucose biosensors using conducting polymers of
4-(4H-dithyinol{[}3,2-b:2',3'-d]pyro11-4-yl)aniline and
4-(4H-dithyinol{[}3,2-b:2',3'-d]pyroll-4-yl)-{[}1,1'-biphenyl]-4-amine
is proposed. After the synthesis and characterizations of the
dithionepyrrole type monomers, they were used in glucose biosensing
applications. The surface of gold ellectrode was modified with
mercaptoethane sulfonic acid and paminothiophenol functionalized gold
nanoparticles and aniline modified GOx. Electrochemical measurements
were carried out by following the consumed oxygen due to the enzymatic
reaction of glucose oxidase. DTP-PhNH2/AuNP/GOx and
DTP-Ph-Ph-NH2/AuNP/GOx biosensors showed very good linearity between 0.1
and 2.5 mM, and 0.05 and 1 mM for glucose, respectively. LOD value was
obtained for pDTP-Ph-NH2/AuNP/GOx as 5.00 x 10(-2) mM and for
pDTP-Ph-Ph-NH2/AuNP/GOx as 9.86 x 10(-5) mM using S/N ratio.
Optimization of molar ratio amount of AuNP/GOx, cycles amount to
immobilize AuNP/GOx, conducting polymer thickness were examined.
Finally, under optimized conditions, the amount of glucose in spiked
human serum samples and recovery experiments were conducted. (C) 2016
Elsevier B.V. All rights reserved.... Show more Show less