Novel amperometric xanthine biosensor based on xanthine oxidase immobilized on electrochemically polymerized 10{[}4H-dithieno(3,2-b:2 `,3 `-d)pyrrole-4-yl] decane-1-amine film
Dervisevic, Muamer and Dervisevic, Esma and Azak, Hacer and Cevik, Emre
and Senel, Mehmet and Yildiz, Huseyin Bekir
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Abstract
In this paper, a novel amperometric xanthine (X) biosensor is
constructed by xanthine oxidase (XOx) immobilization on the pencil
graphite electrode (PGE). Xanthine oxidase is immobilized using
glutaraldehyde (GA) on the electrochemically polymerized conducting
polymer film. The detection of xanthine is based on its consumed amount
due to the enzymatic reaction of xanthine oxidase. The effects of
polymer thickness, applied potential, pH, and temperature were
investigated and optimum parameters weere found to be five cycles, +5V,
+0.5V and 30 degrees C, respectively. Storage stability, operation
stability of the enzyme electrode, and effect of interferant substances
on the amperometric response were also studied. In order to verify the
applicability of proposed biosensor, fabricated electrode was used to
measure the xanthine concentration in chicken meat samples. The present
xanthine biosensor with high selectivity, sensitivity, and stability is
promising for practical applications. (C) 2015 Elsevier B.V. All rights
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