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Calixarene modified montmorillonite: a novel design for biosensing applications

Sonmez, Burak and Sayin, Serkan and Yalcinkaya, Esra Evrim and Seleci, Didem Ag and Yildiz, Huseyin Bekir and Demirkol, Dilek Odaci and Timur, Suna
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Date
2014
URI
http://hdl.handle.net/20.500.12498/4795
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Abstract
Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH2) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH2 modified Mt (Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a CalixNH(2)/ Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail....  Show more  Show less
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