Calixarene modified montmorillonite: a novel design for biosensing applications
Sonmez, Burak and Sayin, Serkan and Yalcinkaya, Esra Evrim and Seleci,
Didem Ag and Yildiz, Huseyin Bekir and Demirkol, Dilek Odaci and Timur,
Suna
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Abstract
Here we report the synthesis, characterization and application of
calixarene (Calix) modified montmorillonite (Mt) as a platform for
bio-applications such as biomolecule immobilization and biosensing
technologies. This modification enhanced the biomolecule immobilization
capability of Mt. Initially, amino-functionalised calixarenes
(Calix-NH2) were synthesized and used as a modifier. X-ray diffraction,
Fourier transform infrared spectroscopy, zeta potential and thermal
gravimetric analysis were performed to verify the modification of the
clay minerals. For the biosensor construction, Calix-NH2 modified Mt
(Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and
pyranose oxidase were immobilized on the surface of a glassy carbon
electrode which was then referred to as a CalixNH(2)/ Mt/PyOx biosensor.
After optimization of the enzyme amount and pH, analytical
characteristics were investigated in detail.... Show more Show less