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dc.contributor.authorGÜLBAHÇE MUTLU, Elif
dc.contributor.authorARSLAN, Emine
dc.contributor.authorÖZNURLU, Yasemin
dc.contributor.authorÖZPARLAK, Haluk
dc.date.accessioned2019-07-09T12:54:31Z
dc.date.available2019-07-09T12:54:31Z
dc.date.issued2018-04-25
dc.identifier.urihttps://hdl.handle.net/20.500.12498/877
dc.description.abstractMany types of aflatoxin cause problems for both public and animal health. Aflatoxin B1 (AFB1) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB1 decreases feed quality, egg production and fertility of hatching eggs. Also, AFB1 alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB1 on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation. DNA isolated from the liver of chick embryos treated with different concentrations of AFB1 was separated using agarose gel electrophoresis to detect apoptosis, and DNA interaction with AFB1 was investigated using plasmids to detect changes in electrophoretic mobility and their effects on DNA. Base changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and 40 ng/egg doses of AFB1 were increased on day 19 compared to base changes of the same AFB1 doses on day 13. We also found that AFB at different concentrations changed the mobility of DNA by binding to it, and that high doses of AFB1 destroyed DNA. The DNA interaction study using plasmid demonstrated that AFB1 at high doses was bound to plasmid DNA, slowed its mobility and inhibited restriction cuts.en_US
dc.language.isoenen_US
dc.publisherBiotechnic & Histochemistryen_US
dc.subjectDNA Interactionen_US
dc.subjectAflatoxin B1en_US
dc.subjectBroiler Chickensen_US
dc.subjectGrowth Hormone Regulated Gene-1en_US
dc.subjectSequence Analysisen_US
dc.titleThe effects of aflatoxin B1 on growth hormone regulated gene-1 and interaction between DNA and aflatoxin B1 in broiler chickens during hatchingen_US
dc.typeMakaleen_US


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