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dc.contributor.authorYücel, K
dc.contributor.authorAbusoğlu, S
dc.contributor.authorUnlü, A
dc.date.accessioned2019-07-09T13:48:49Z
dc.date.available2019-07-09T13:48:49Z
dc.date.issued2016-09-01
dc.identifier.urihttps://hdl.handle.net/20.500.12498/922
dc.description.abstractIntroduction: Excessive production of androstenedione can becaused by defects of adrenal steroid biosynthesis, tumors of ovarian and adrenal origin, polycystic ovarian syndrome, increased peripheral sensitivity to androgens, and increased peripheral production of androgens. Most epidemiologic studies use enzyme-linked immunosorbentassay (ELISA) to measure sex steroid hormones because they have acceptable turnaround times and arerelatively inexpensive. Mass spectrometry-based methods are currently the most specific quantitative analytical methods for steroid determination. Mass spectrometry methods are independent of matrix effects or cross-reactivity. In this study, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed. Materials and Methods: For serum androstenedione measurement, 50 lL of internal standard (d5-11 deoxycortizol) in methanol was added to 250 lL standart or serum and centrifuged at 4.500 rpm for 10 minutes to remove the precipitated proteins. Supernatant was transferred to clean tubes and this procedure was performed twice. The supernatant was collected and dried under a nitrogen gas flow at 60 ◦C and dissolved in mobile phase. 60 lL was injected in to the ultra performance liquid chromatography analytical column for chromatography. ELISA study was conducted with DRG (Lot. No. 50K074) brand kit. Results: Method comporison between LC-MS/MS and ELISA was found slope value 18,412, intercept value 22.87 and r² value 0.1033. The regressione quation was ELISA= 2.861782 + 4.905103 LC-MS/MS. Discussion and Conclusion: Method comparison study presented higher results in ELISA compared to LC-MS/MS. In our opinion, this might due to the interference in ELISA systems. Our LC-MS/MS method allows rapid, sensitive and specific determination of androgens in plasma and serum.The specificity of liquid chromatography–tandem mass spectrometry (LC-MS/ MS) offers advantages over immunoassays.en_US
dc.titleComparison of immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods in the measurement of serum androstenedione levelsen_US


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