The Effect on β-Catenin Gene Expression of Grape Seed Extract in Experimental Diabetic Rats

View/ Open
Date
2016-05-23Author
GÜLBAHÇE MUTLU, Elif
ARSLAN, Emine
ARIKOĞLU, Hilal
Metadata
Show full item recordAbstract
Aim of the study: Diabetes Mellitus (DM) a chronic metabolic disease which occured as a result of
deterioration in the signaling pathways of insulin in the target tissues. And, this case continued throughout
the patient's life and reduced quality of life. WNT pathway is involved in lipid metabolism and glucose
homeostasis, and β-catenin is located in this patway. In this study, we aimed to investigate the effects of
grape seed (Vitis vinifera L.) extracts on β-catenin gene expression in wistar rats created experimental
diabetic.
Material and Methods: Rats were divided into five groups; healthy control group, diabetic control group,
100mg/kg, 200mg/kg and 400mg/kg extracts given the treatment groups. Methanol extract of Vitis vinifera
was administered orally for 4 weeks. The β-catenin gene expression levels were determined by
quantitative Real Time-Polymerase Chain Reaction (Real Time-PCR) method in liver and pancreas of
rats.The statistical analysis was performed and One-way ANOVA (Tukey) was used to test for differences
between groups.
Results: The results of β-catenin gene expression obtained from the liver and pancreas tissue were
determined no significant difference among the groups (P>0.05). Our study results showed that the
administered treated doses of seed extracts have not any effect on β-catenin gene expression in these
tissue.
Acknowledgements: The study was supported by grants from the Coordination Committee of Scientific
Research Projects of Selçuk University (BAP, no:13201030).
Collections

DSpace@Karatay by Karatay University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..