Cerebral Ischemia Reperfusion Injury in Rats: Effect of 3’,4’-Dihydroxyflavonol

Abstract

The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on experimental brain ischemia-reperfusion in rats. The study was performed on the 34 male Wistar-albino rats, weigth 350-400 gr. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; 3-DiOHF+Ischemiareperfusion; 4-Ischemia+DiOHF+Reperfusion; 5- Ischemia-reperfusion+DiOHF. DiOHF was given to animals as 10 mg/kg by intraperitoneal. Cerebral cortex was analyzed for malondyaldehyde (MDA), NO (nitric oxide), xanthine oxidase (XO), glutathione (GSH) and glutathione peroxidase (GPx). Tissue MDA levels were significantly higher ischemia-reperfusion groups (p<0.005). However, DiOHF inhibited MDA. Ischemiareperfusion led to increased XO and NO but DiOHF supplementation reduced NO and XO. DiOHF increased GSH and GPx levels compared to ischemia-reperfusion group. Our present study showed that intraperitoneal DiOHF supplementation has protective effect on brain ischaemi-reperfusion injury in rat.