Folic-Acid-Modified Conducting Polymer: Electrochemical Detection of the Cell Attachment.

  • Yazar/lar AZAK, Hacer
    BARLAS, Fırat Barış
    YILDIZ, Hüseyin Bekir
    GÜLEÇ, Kadri
    DEMİR, Bilal
    ODACI DEMİRKOL, Dilek
    TİMUR, Suna
  • Yayın Türü Makale
  • Yayın Tarihi 2016
  • DOI Numarası 10.1002/mabi.201500274
  • Tek Biçim Adres http://hdl.handle.net/20.500.12498/3343

Here, postfunctionalization and bioapplication of a π-conjugated polymer named 4-[4H-dithieno(3,2-b:2',3'-d)pyrrol-4-yl]aniline (DTP-aryl-NH2 ) are reported, which is successfully synthesized via electropolymerization onto the glassy carbon electrode. Folic acid (FA) is used to modify the amino functional polymer via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide chemistry for the further steps. The selective adhesion of folate receptor positive cells on the surface is followed by the electrochemical methods. Cyclic voltammetry and electrochemical impedance spectroscopy have been used to characterize stepwise modification of the electroactive surface. After optimization studies such as scan rate during the polymer deposition, FA amount for the efficient surface targeting, incubation time with the cells etc., analytical characterization is carried out. The surface morphologies at each step are imaged by using fluorescence microscopy.

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Eser Adı
(dc.title)
Folic-Acid-Modified Conducting Polymer: Electrochemical Detection of the Cell Attachment.
Yayın Türü
(dc.type)
Makale
Yazar/lar
(dc.contributor.author)
AZAK, Hacer
Yazar/lar
(dc.contributor.author)
BARLAS, Fırat Barış
Yazar/lar
(dc.contributor.author)
YILDIZ, Hüseyin Bekir
Yazar/lar
(dc.contributor.author)
GÜLEÇ, Kadri
Yazar/lar
(dc.contributor.author)
DEMİR, Bilal
Yazar/lar
(dc.contributor.author)
ODACI DEMİRKOL, Dilek
Yazar/lar
(dc.contributor.author)
TİMUR, Suna
DOI Numarası
(dc.identifier.doi)
10.1002/mabi.201500274
Atıf Dizini
(dc.source.database)
Diğer
Atıf Dizini
(dc.source.database)
Pubmed
Yayın Tarihi
(dc.date.issued)
2016
Kayıt Giriş Tarihi
(dc.date.accessioned)
2020-08-07T13:24:46Z
Açık Erişim tarihi
(dc.date.available)
2020-08-07T13:24:46Z
Kaynak
(dc.source)
Macromolecular bioscience
Özet
(dc.description.abstract)
Here, postfunctionalization and bioapplication of a π-conjugated polymer named 4-[4H-dithieno(3,2-b:2',3'-d)pyrrol-4-yl]aniline (DTP-aryl-NH2 ) are reported, which is successfully synthesized via electropolymerization onto the glassy carbon electrode. Folic acid (FA) is used to modify the amino functional polymer via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide chemistry for the further steps. The selective adhesion of folate receptor positive cells on the surface is followed by the electrochemical methods. Cyclic voltammetry and electrochemical impedance spectroscopy have been used to characterize stepwise modification of the electroactive surface. After optimization studies such as scan rate during the polymer deposition, FA amount for the efficient surface targeting, incubation time with the cells etc., analytical characterization is carried out. The surface morphologies at each step are imaged by using fluorescence microscopy.
Yayın Dili
(dc.language.iso)
en
Tek Biçim Adres
(dc.identifier.uri)
http://hdl.handle.net/20.500.12498/3343
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