Comparative Analysis of Agronomic Traits and ISSR Method among Some Soybeans [Glycine Max (L.) Merr.] Genotypes
ARSLAN, Emine; GÜLBAHÇE MUTLU, Elif; DURSUN, Ömer; BAĞCI, S. Ahmet
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Abstract
In this study, the genetic diversity was investigated among 12
soybeans genotypes using inter simple sequence repeats (ISSR) and
agronomic traits. DNA was isolated from the leaves of the genotypes.
For molecular characterization, a total of 26 primers of ISSRs and
eight agronomic characteristics were evaluated. ISSR analysis
revealed 88 polymorphic bands. The genetic diversity among the
genotypes according to ISSR analysis and agronomic traits were
estimated based on Nei homology and Eucllidian distance,
respectively, and dendrograms reflecting genetic similarity were
constructed using UPGMA and NTSYSpc, respectively. Nei’s
homology coefficient values used for ISSR analysis ranged from 78%-
84%, and the average Euclidean distance used for agronomic data
ranged from 1.96-9.77. Although soybean genotypes evaluated in this
study were highly similar, dendrograms showed that these genotypes
could be distinguished both morphologically and genetically.... Show more Show less