Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase

In this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2’,3’d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances, linear range, detection limit, limit of quantification and the Michaelis-Menten constant (Km) of biosensor electrodes were obtained 2.0 mM–23.7 mM, 0.27 mM, 0.82 mM, 17,81 mM respectively. Biosensor optimization parameters: optimum pH, optimum temperature, stability test and response time were evaluated. The real sample and recovery studies were also performed in order to show applicability of the biosensing electrodes

Erişime Açık
Görüntülenme
10
22.03.2024 tarihinden bu yana
İndirme
1
22.03.2024 tarihinden bu yana
Son Erişim Tarihi
24 Nisan 2024 01:17
Google Kontrol
Tıklayınız
Tam Metin
Tam Metin İndirmek için tıklayın Ön izleme
Detaylı Görünüm
Eser Adı
(dc.title)
Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase
Yayın Türü
(dc.type)
Makale
Yazar/lar
(dc.contributor.author)
ÇEVİK, Emre
Yazar/lar
(dc.contributor.author)
CERİT, Alaaddin
Yazar/lar
(dc.contributor.author)
GAZEL, Nilay
Yazar/lar
(dc.contributor.author)
YILDIZ, Hüseyin Bekir
Atıf Dizini
(dc.source.database)
Wos
Atıf Dizini
(dc.source.database)
Scopus
Konu Başlıkları
(dc.subject)
Cholesterol Biosensor
Konu Başlıkları
(dc.subject)
Cholesterol Oxidase
Konu Başlıkları
(dc.subject)
DTP Condusting Polymer
Yayıncı
(dc.publisher)
Electroanalysis (Wiley)
Yayın Tarihi
(dc.date.issued)
2018
Kayıt Giriş Tarihi
(dc.date.accessioned)
2019-07-09T06:17:57Z
Açık Erişim tarihi
(dc.date.available)
2019-07-09T06:17:57Z
Atıf için Künye
(dc.identifier.citation)
DOI: 10.1002/elan.201800248
Özet
(dc.description.abstract)
In this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2’,3’d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances, linear range, detection limit, limit of quantification and the Michaelis-Menten constant (Km) of biosensor electrodes were obtained 2.0 mM–23.7 mM, 0.27 mM, 0.82 mM, 17,81 mM respectively. Biosensor optimization parameters: optimum pH, optimum temperature, stability test and response time were evaluated. The real sample and recovery studies were also performed in order to show applicability of the biosensing electrodes
Yayın Dili
(dc.language.iso)
en
Tek Biçim Adres
(dc.identifier.uri)
https://hdl.handle.net/20.500.12498/724
Analizler
Yayın Görüntülenme
Yayın Görüntülenme
Erişilen ülkeler
Erişilen şehirler
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve cerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms