Construction of ferrocene modified conducting polymer based amperometric urea biosensor

Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by fi rstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The e ff ect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor’s amperometric response was linear in the range of 0.1–8.5 mM with the sensitivity of 0.54 μA/mM, detection limit of 12 μM, and short response time of 2 s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insigni fi cant interference.

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Eser Adı
(dc.title)
Construction of ferrocene modified conducting polymer based amperometric urea biosensor
Yayın Türü
(dc.type)
Makale
Yazar/lar
(dc.contributor.author)
DERVISEVIC, Muammer
Yazar/lar
(dc.contributor.author)
DERVISEVIC, Esma
Yazar/lar
(dc.contributor.author)
ŞENEL, Mehmet
Yazar/lar
(dc.contributor.author)
ÇEVİK, Emre
Yazar/lar
(dc.contributor.author)
YILDIZ, Hüseyin Bekir
Yazar/lar
(dc.contributor.author)
ÇAMURLU, Pınar
Atıf Dizini
(dc.source.database)
Wos
Atıf Dizini
(dc.source.database)
Scopus
Konu Başlıkları
(dc.subject)
Urea
Konu Başlıkları
(dc.subject)
Urease
Konu Başlıkları
(dc.subject)
Amperometric Biosensor
Konu Başlıkları
(dc.subject)
SNS Aniline
Konu Başlıkları
(dc.subject)
Ferrocene
Yayıncı
(dc.publisher)
Enzyme And Microbial Technology (Elsevier)
Yayın Tarihi
(dc.date.issued)
2017
Kayıt Giriş Tarihi
(dc.date.accessioned)
2019-07-09T06:23:56Z
Açık Erişim tarihi
(dc.date.available)
2019-07-09T06:23:56Z
Özet
(dc.description.abstract)
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by fi rstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The e ff ect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor’s amperometric response was linear in the range of 0.1–8.5 mM with the sensitivity of 0.54 μA/mM, detection limit of 12 μM, and short response time of 2 s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insigni fi cant interference.
Yayın Dili
(dc.language.iso)
en
Tek Biçim Adres
(dc.identifier.uri)
https://hdl.handle.net/20.500.12498/729
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