The effects of aflatoxin B1 on growth hormone regulated gene-1 and interaction between DNA and aflatoxin B1 in broiler chickens during hatching

Many types of aflatoxin cause problems for both public and animal health. Aflatoxin B1 (AFB1) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB1 decreases feed quality, egg production and fertility of hatching eggs. Also, AFB1 alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB1 on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation. DNA isolated from the liver of chick embryos treated with different concentrations of AFB1 was separated using agarose gel electrophoresis to detect apoptosis, and DNA interaction with AFB1 was investigated using plasmids to detect changes in electrophoretic mobility and their effects on DNA. Base changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and 40 ng/egg doses of AFB1 were increased on day 19 compared to base changes of the same AFB1 doses on day 13. We also found that AFB at different concentrations changed the mobility of DNA by binding to it, and that high doses of AFB1 destroyed DNA. The DNA interaction study using plasmid demonstrated that AFB1 at high doses was bound to plasmid DNA, slowed its mobility and inhibited restriction cuts.

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Eser Adı
(dc.title)
The effects of aflatoxin B1 on growth hormone regulated gene-1 and interaction between DNA and aflatoxin B1 in broiler chickens during hatching
Yayın Türü
(dc.type)
Makale
Yazar/lar
(dc.contributor.author)
GÜLBAHÇE MUTLU, Elif
Yazar/lar
(dc.contributor.author)
ARSLAN, Emine
Yazar/lar
(dc.contributor.author)
ÖZNURLU, Yasemin
Yazar/lar
(dc.contributor.author)
ÖZPARLAK, Haluk
Atıf Dizini
(dc.source.database)
Wos
Atıf Dizini
(dc.source.database)
Scopus
Konu Başlıkları
(dc.subject)
DNA Interaction
Konu Başlıkları
(dc.subject)
Aflatoxin B1
Konu Başlıkları
(dc.subject)
Broiler Chickens
Konu Başlıkları
(dc.subject)
Growth Hormone Regulated Gene-1
Konu Başlıkları
(dc.subject)
Sequence Analysis
Yayıncı
(dc.publisher)
Biotechnic & Histochemistry
Yayın Tarihi
(dc.date.issued)
2018
Kayıt Giriş Tarihi
(dc.date.accessioned)
2019-07-09T12:54:31Z
Açık Erişim tarihi
(dc.date.available)
2019-07-09T12:54:31Z
Özet
(dc.description.abstract)
Many types of aflatoxin cause problems for both public and animal health. Aflatoxin B1 (AFB1) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB1 decreases feed quality, egg production and fertility of hatching eggs. Also, AFB1 alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB1 on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation. DNA isolated from the liver of chick embryos treated with different concentrations of AFB1 was separated using agarose gel electrophoresis to detect apoptosis, and DNA interaction with AFB1 was investigated using plasmids to detect changes in electrophoretic mobility and their effects on DNA. Base changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and 40 ng/egg doses of AFB1 were increased on day 19 compared to base changes of the same AFB1 doses on day 13. We also found that AFB at different concentrations changed the mobility of DNA by binding to it, and that high doses of AFB1 destroyed DNA. The DNA interaction study using plasmid demonstrated that AFB1 at high doses was bound to plasmid DNA, slowed its mobility and inhibited restriction cuts.
Yayın Dili
(dc.language.iso)
eng
Tek Biçim Adres
(dc.identifier.uri)
https://hdl.handle.net/20.500.12498/877
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