Comparison of real-time PCR, indirect immunofluorescence antibody assay, and different staining techniques for the diagnosis of Pneumocystis jirovecii

DURGUT, Elif Gözde | KUŞTİMUR, Semra | AYDIN TERZİOĞLU, Merve | KALKANCI, Ayşe | DOĞRUMAN AL, Funda

Aim: Pneumocystis pneumonia (PCP), caused by Pneumocystis jirovecii (P. jirovecii), is an opportunistic infection with a severe progression, often observed in immunocompromised patients. The diagnosis is difficult due to the non-specific clinical and radiological findings. Therefore, rapid and accurate diagnosis of the agent is important in terms of timely implementation of the treatment. In this study, it was aimed to detect P. jirovecii by Giemsa staining, Modified Toluidine Blue O staining (MTolB), indirect immunofluorescent antibody (IIFA) assay, and real-time polymerase chain reaction (PC ...Daha fazlası

Makale 2020 Annals of Medical Research 6 Görüntülenme

Investigation of antifungal, antibiofilm and antifilamentation activities of biocides against Candida isolates

ÖZTÜRK, Ali | ABDULMAJED, Olkar | AYDIN TERZİOĞLU, Merve

Aim: This study was aimed to evaluate the antifungal, antibiofilm, and anti-filamentation activities of three commonly used biocides against clinical Candida isolates. Material and Methods: The in vitro activities of benzalkonium chloride (BZC), chlorhexidine digluconate (CHX), and triclosan (TRC) were studied against three Candida (C. albicans, C. parapsilosis, and C. glabrata) isolates. The isolates were identified, using the germ tube test, Cornmeal agar, and ID32 C (bioMerieux, France) kit. Antifungal susceptibility of these isolates was determined using the broth microdilution method. Ant ...Daha fazlası

Makale 2021 Annals of Medical Research 6 Görüntülenme