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Identification of medically important yeasts by sequence analysis of the internal transcribed spacer and D1/D2 region of the large ribosomal subunit

AYDIN, Merve | KUŞTİMUR, Semra | KALKANCI, Ayşe | DURAN, Tuğçe

Background: The prevalence of opportunistic yeast infections has increased in recent decades as the resultof an increasing immunocompromised patient population.Aims: To evaluate ribosomal RNA (rRNA) gene sequence to identify medically important yeast species, toinvestigate the performance of both the rRNA gene internal transcribed spacer (ITS) and D1/D2 region in identifying clinically relevant yeasts, and to compare these results with those of a standard phenotypicmethod.Methods: Both regions from 50 yeast strains, comprising 45 clinical isolates and 5 reference strains, wereamplified using P ...Daha fazlası

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Molecular characterization and subtyping of Blastocystis in urticarial patients in Turkey

AYDIN, Merve | YAZICI, Mustafa | DEMİRKAZIK, Mehtap | KOLTAŞ, İsmail Soner | ÇIKMAN, Aytekin | GÜLHAN, Barış | DURAN, Tuğçe | YILMAZ, Aysun | KARA, Murat

Objective: To investigate Blastocystis’ etiologic role and association with gastrointestinal symptomatology in acute and chronic urticaria patients and to identify Blastocystis subtypes responsible for urticaria. Methods: The study included urticaria patients and healthy individuals that presented to our polyclinic between June 2015 and May 2017. The participants were assigned into Group I (137 patients), subdivided into acute (72) and chronic urticaria patients (65), and Group II (129 control individuals). Blastocystis presence was investigated by native-Lugol examination, trichrome staining, ...Daha fazlası

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Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

TOKAK, Semih | KILIÇ, İbrahim Halil | YALÇIN, Hüsniye Tansel | DURAN, Tuğçe

Yeasts are usually found in high amounts in dairy products, which show their ability to adapt to substrates rich in protein, lipid. sugar and organic acids. A wide distribution of yeast in dairy products is a result of proteolytic and lipolytic is activities. Spoilage yeasts and molds can grow in most. processed and raw limits. where environmental conditions are not suitable for most bacteria (low pH low water activity, aw). Nutrients and oxygen in food are the main factors that determine the type of fungal spoilage. In this study, dairy products samples (yoghurt, cream. butter, curd cheese. A ...Daha fazlası

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Effects Of Fentanyl On Pancreatic Cancer Cell Proliferation And Cancer Stem Cell Differentiation

ÇELİK, Feyzi | DURAN, Tuğçe

Pancreatic cancer is one of the most aggressive cancer due to the late diagnosis and failure to respond to the treatment despite advances in tumor biology and the development of new cancer therapeutic strategies. It has been reported that these characteristics of pancreatic cancer originate from cancer stem cells within the tumor mass. It has also been reported that Fentanyl is a fast-acting analgesic that binds to the mu-opioid receptors and some other mu-opioid receptors are involved in this cancer process. In this study, we determined the effect of Fentanyl on PANC-1 cells, by assessing the ...Daha fazlası

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Fentanyl Induces the Differentiation of Cancer Stem Cell and Apoptosis

YILDIRIM, İbrahim Halil | DURAN, Tuğçe

Cancer stem cells are involved in the onset and development of cancer as well as in resistance to chemo and radiotherapy and also metastasis. Elimination of these cells or inducing to differentiation to normal cancer cells will be important in the treatment of cancer. There are some publications that report the positive effects of mu-opioid antagonists on cancer stem cell differentiation. Fentanyl is a mu-opioid analgesic that acts 80 times faster than morphine and is frequently used in cancer patients. In this study, we treated the pancreatic cancer cells, BxPC-3 with Fentanyl and compared th ...Daha fazlası

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